Bio-Rad Bole GenepulserxcellHiki i ka ʻōnaehana electroporation ke hoʻokomo i nā nucleotides, nā DNA i ka RNA, nā proteins, glycans, dyes, a me nā ʻāpana viral, a me nā mea like i loko o nā cell prokaryotic a me eukaryotic.
ʻO ka electroporation kahi ʻenehana maikaʻi e hiki ai ke hoʻokomo i nā ʻakika nucleic, nā protein, a me nā molekole ʻē aʻe i loko o nā ʻano mea like ʻole. Ma o ke kahua uila ikaika kiʻekiʻe, e hoʻonui koke i ka permeability o ka membrane cell, a laila e hoʻomoʻa i kahi mole exogenous i loko o ke kikowaena a puni. Hiki i kēia ʻenehana ke hoʻokomo i nā nucleotides, DNA i ka RNA, protein, glycos, dye, a me nā ʻāpana viral, a me nā mea like. ʻO ka hoʻololi uila e pili ana i nā ʻano hoʻololi kino a me nā mea hoʻololi kemika he mea waiwai a maikaʻi hoʻi. ʻO ka hoʻolālā ʻōnaehana Gene Pulserxcell, e pili ana i ka Bio-RAD15, luhi ia i ka ʻenehana electrohydration, e hāʻawi ana i ka nalu kuhikuhi a me ke koho ʻano nalu square, koho hoʻonohonoho hoʻonohonoho ʻōnaehana, a me ka mea hoʻohana aloha.
Bio-Rad Bole GenepulserxcellNā hiʻohiʻona o ka ʻōnaehana uʻi uila:
1, ka nalu kuhikuhi a me ke ʻano hawewe huinahā e hōʻoia i nā ʻano cell āpau (prokaryotes a me eukaryotic) hiki ke hoʻokō i nā hopena hoʻololi uila maikaʻi loa;
2, Bio-Rad patented PulsetRAC circuit a me ka hoʻolālā pale arc e hōʻoia i ka hana hou a pale i nā laʻana;
3, hiki i ka hoʻolālā modular ke koho i ka ʻōnaehana, ka hoʻohana ʻana i ke kikowaena kikohoʻe, me ka hoʻolālā intuitive e hoʻokele i nā ʻāpana āpau, me nā ʻāpana o ka module accessory. Me ka koho papahana o ka hana lima, nā kaʻina hana preset, nā kaʻina hana hoʻohana, kahi kaʻina hana optimization a me nā hana holomua ʻē aʻe;
4, US Patent 4,750,100 a me 4,910,140.
Bio-Rad Bole Genepulserxcell
Ua hui pū ʻia ʻo Gene Pulser Xcell Loaʻa i ka ʻāpana nui, ka module CE, ka module PC | |
Hoʻopuka | Ka nalu: Pohō helu helu a i ʻole ka nalu huinahā; ka uila: 10-3,000 V |
Ka manaʻo | 10-500 V, 25-3, 275 μF, 25 μF hoʻonui 500-3,000 V, 10, 25, 50 μF |
Kū'ē (parallel) | 50-1,000 Ω, hoʻonui 50 Ω, pau ʻole |
Laʻana pale | 10-2, 500 V, 20 Ω ka palena iki 2,500-3,000 V, 600 Ω ka palena iki |
Ka manawa nalu huinaha | 10-500 V: Ka lōʻihi 0.05-10 ms, 0.05 ms hoʻonui; ʻO ka lōʻihi 10-100 ms, hoʻonui 1 ms, 1-10 mau puʻupuʻu, waena 0.1-10 s 500-3,000 V: Ka lōʻihi 0.05-5 m. |
Gene Pulser Xcell Qual System Loaʻa i ka ʻāpana nui, CE module; | |
Hoʻopuka a me nā kiko'ī'ē aʻe | Ma waho aʻe o ka mana kū'ēʻole, hoʻopuka a like paha me ka pūnaewele piha |
Gene Pulser Xcell Microbiological System | |
Loaʻa nā mea nui, nā modula PC | |
Hoʻopuka | Ka nalu: Pohō helu a i ʻole ka nalu huinahā Volta: 200-3,000 V |
Ka manaʻo | 10, 25, 50 μF |
Kū'ē (parallel) | 50-1,000 Ω hoʻonui 50 Ω, hoʻohui i ka palena ʻole |
Laʻana pale | 200-2, 500 V, 20 Ω ka palena iki 2,500-3,000 V, 600 Ω ka palena iki |
Ka manawa nalu huinaha | Ka lōʻihi 0.05-5 m. |
Gene Pulser XCell hui nui | |
Hoʻopuka | Ka nalu: Pohō a i ʻole ka huinahā Volta: 200-3,000 V |
Kāpena hoʻokuʻu | 10, 25, 50 μF |
Laʻana pale | 200-2, 500 V, 20 Ω ka palena iki 2,500-3,000 V, 600 Ω ka palena iki |
Ka manawa nalu huinaha | Ka lōʻihi 0.05-5 m. |
Kuʻuna | |
Volta hoʻokomo | 100-120 VAC a i ʻole 220-240 VAC, 50/60 HZ |
Lako ikehu | 240 w kiʻekiʻe loa (i ke kaʻina hoʻokuʻu pōkole) |
Kaiapuni Hana | ʻO ka mahana 0-35 ° C, haʻahaʻa 0-95% (ʻaʻohe anu) |
Palapala palekana | EN61010, EMC EN61326 A pae A hōʻoia palekana |
Nui (wxdxh) | ʻĀpana nui: 31 x 30 x 14 cm; CE module: 31 x 28 x 9 knm; Kōpae PC: 31 x 28 x 5 knm |
Kaumaha | Hui nui: 6.6 kg; CE module: 3.1 kg; PC module: 1.9 kg |