CFX96TOUCH Fluorescent Quantitative PCR mea kani

Hiki ke hoʻohana ʻia ka CFX96Touch fluorescent quantitative PCR i nā ʻano noiʻi like ʻole o ka helu nucleic acid quantification, gene expression level analysis, gene mutation detection, GMO detection, and product specific analysis.

Kaiapuni hana:
1.1 Ka wela hana: 5-31 ° C
1.2 Ka hana a me ka haʻahaʻa: pili ≤80%
1.3 Mana hana: 100-240 VAC, 50-60Hz
ʻO ka hana a me nā koi ʻenehana o CFX96Touch fluorescence quantitative PCR
3.1 Hana nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia)
* 3.1.1 ʻEono mau ala hoʻāʻo, 5% PCR hiki ke hoʻokō ʻia, a hiki ke ʻike ʻia nā genes target 5 i ka manawa like, a ʻike ʻia ke kahawai ʻike FRET kūikawā i ka manawa like.
* 3.1.2 Me ka hana PCR gradient mahana, hiki iā ʻoe ke holo i 8 mau mahana like ʻole i ka manawa like, kēlā me kēia wela incubation.
3.1.3 Ka wehe 'ana i nā reagents piha, ho'ohana 'ia nā noi'i like 'ole a me nā lā'au lapa'au;
3.1.4 He kūpono no nā ʻano hana fluorescence e like me Taqman, Molecular Beacon, Fret probe, Sybr Green i, etc.;
3.1.5 Wehe, 0.2ml paipu hoʻokahi, octal, 96-well pā, etc.
* Hiki i ka 3.1.6 ke holo kūʻokoʻa, ʻoiaʻiʻo ma waho o ka hana, ʻaʻohe pono e hoʻopili i ke kamepiula e nānā i ka PCR fluorescent amplification curve i ka manawa maoli;
3.2 Nā koi ʻenehana nui (* ʻo ia ka hōʻailona e pono e hoʻokō ʻia)
* 3.2.1 Hiki ke hoʻohālikelike: 96 × 0.2ml, nā kikoʻī maʻamau 96-well plates (12 × 8) hiki ke hoʻohana ʻia;
3.2.2 Mea lako: 0.2ml paipu hoʻokahi, ʻewalu pili, 96-well papa, etc.
3.2.3 Pūnaehana Reaction: 1-50μL (manaʻo ʻia 10-25 μL);
* 3.2.4 Puna kukui: ʻEono mau LED me nā kānana;
* 3.2.5 ʻIke: ʻEono diodes photosensitive me nā kānana;
* 3.2.6 lita hoʻomaha māmā: 5 ° C / sec;
3.2.7 Manaʻo hoʻomalu wela: 0 -100 ° C;
3.2.8 Pono Pono: ± 0.2 ° C (90 ˚C);
3.2.9 Kaulike mahana: ± 0.4 ° C (90 ˚C i loko o 10 kekona);
* 3.2.10 Hana ʻia ʻo Dynamic Temperature Gradient Function: Holo i 8 mau mahana like ʻole i ka manawa like; gradient ka mana o ka wela: 30 -100 ° C; ʻokoʻa ʻokoʻa o ka mahana gradient: 1 - 24 ° C; gradient mahana incubation manawa: like;
3.2.11 hoʻohiwahiwa / hoʻokuʻu ʻia ka lōʻihi hawewe: 450-730 nm;
3.2.12 ʻIke: Hiki ke ʻike ʻia ke ʻano kope kope hoʻokahi i ka genome kanaka;
3.2.13 Ka laulā dynamic: 10 nui;
3.2.14 Hōʻike: 8.5 iniha kala hoʻopā pale;
3.2.15 Ke Ana Ikepili Ka Ikepili: Ka Nui Curve ma'amau, Melting Curve, CT or ΔΔCT Gene Expression Analysis, Multiple Internal Genodes Analysis and Amplification Efficiency Calculation, Multiple Data Files Gene Expression Analysis, Allelic Analysis, Type Analysis, Have Gene, melting curve analysis function ;
3.2.16 Hoʻouna ʻikepili: Excel, Word, a i ʻole PowerPoint. Aia i loko o ka hōʻike mea hoʻohana nā hoʻonohonoho holo, nā kiʻi, a me nā hopena ʻikepili papa, hiki ke paʻi ʻia a mālama ʻia paha ma PDF;
* 3.2.17 Nā haʻawina hoʻonaʻauao Chromosomal: He ʻano o ka nānā ʻana i ka nui o nā hale chromatin ma o ka hana hoʻohālikelike o ka hoʻohaʻahaʻa DNA genomic ma o ka hana hoʻohālikelike o ka DNA genomic. Hōʻoiaʻiʻo ia i ka pilina kiʻekiʻe ma waena o ke ʻano chromatin a me ka hōʻike gene;